cpg oligodeoxynucleotide Search Results


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Novus Biologicals tlr9
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
Cpg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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Bio-Techne corporation cpg
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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TIB MOLBIOL completely phosphorothioate-modified cpg-oligodeoxynucleotide (odn) 1668 (tcc atg acg ttc ctg at)
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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TriLink purified ps oligodeoxynucleotides cpg 22-mer cpg-ps-dna
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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Midland Certified Reagent phosphorothioate cpg oligodeoxynucleotide
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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TIB MOLBIOL cpg 1826
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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MWG-Biotech ag cpg-oligodeoxynucleotide 2216
Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), <t>TLR9</t> (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.
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Image Search Results


Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), TLR9 (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.

Journal: The Journal of infectious diseases

Article Title: Hepatitis C Virus nonstructural 5A protein inhibits lipopolysaccharide-mediated apoptosis of hepatocytes by decreasing expression of Toll-like receptor 4.

doi: 10.1093/infdis/jir381

Figure Lengend Snippet: Figure 1. Hepatitis Cvirus (HCV) nonstructural protein 5 A (NS5A) protects hepatocytes from lipopolysaccharide (LPS)–induced cell death. A, B, HepG2 control (A) and HepG2-NS5A (B) hepatocytes were cultured for 24 hours with ligands of Toll-like receptor (TLR) 1/TLR2 (Pam3CSK4), TLR3 (poly[I:C]), TLR4 (LPS), TLR5 (flagellin), TLR6/TLR2 (macrophage-activating lipopeptide 2), TLR7 (Imiquimod [R-837]), TLR9 (type B CpG oligonucleotide), and control oligonucleotide, as indicated in Materials and Methods. Cells were washed and stained with crystal violet, and experiments were performed 3 times. PBS, phosphate-buffered saline. C, HCV NS5A protects hepatocytes from LPS-induced apoptosis. HepG2 control and HepG2-NS5A hepatocytes were cultured for 24 hours with LPS (5 lg/mL). Cell apoptosis was quantified using the APOPercentage Apoptosis Assay. Data are expressed as means 6 standard deviations of triplicate determinations from 1 experiment represen- tative of 3 independent experiments.

Article Snippet: 3HCL; 100 lg/mL), TLR3 (poly[I:C]; 50 lg/mL), TLR4 (LPS from Escherichia coli; 5 lg/mL), TLR5 (purified flagellin; 100 lg/mL), TLR6/TLR2 (macrophage-activating lipopeptide 2; 100 lg/mL), TLR7 (Imiquimod [R-837]; 2.5 lg/mL), and TLR9 (type B CpG ODN; 0.5 lg/mL) (all purchased from Imgenex).

Techniques: Control, Cell Culture, Staining, Saline, Apoptosis Assay